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Determination of TauroLock stability
Matejčíková, Eva ; Mravcová, Ludmila (referee) ; Čáslavský, Josef (advisor)
This diploma thesis deals with the study of the anticoagulant and antimicrobial agent TauroLockTM, which is manufactured by the german company TauroPharm GmbH. Based on the background research and taking into account the equipment of analytical laboratories of the Institute of Chemistry and Technology of Environmental Protection, optimal methods of analysis were proposed and a series of experiments were carried out which focused on the determination of the active substance and degradation products of taurolidine in TauroLockTM which was stored for a certain period of time in real conditions. The main task of this work was to determine the presence of taurolidine as the active substance and formaldehyde as the undesirable product of its decomposition.
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Fixation of the biological samples
Brachtl, Martin ; Jiřík, Radovan (referee) ; Krátká, Lucie (advisor)
The aim of thesis is acquaint with possibilities of fixation biological tissues. Choose a suitable tissue and fix it with formaldehyde and another fixator. Last but not least make measurements which determine effects of formaldehyde on the relaxation time of biological tissues. Mice brains were chosen as a suitable for tissues fixation. Measuring effects of formaldehyde and another fixator to relaxation time carried out at the Institute of Scientific Instuments Academy of Sciences of the Czech Republic.
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Voltammetry with Preliminary Extraction as a New Approach for Rapid Determination of Formaldehyde in Wood-Based Products
Dvořák, Pavel ; Vyskočil, Vlastimil (advisor) ; Dejmková, Hana (referee)
The aim of the presented Diploma Thesis was to develop a new method for the indirect determination of formaldehyde in wood-based products using gas-diffusion microextraction coupled with electrochemical detection on unmodified screen-printed electrodes (MLEM-SPCE). Formaldehyde released from the sample is derivatized using an acetylacetone reagent present in an acceptor solution. The product of derivatization of formaldehyde with acetylacetone is 3,5-diacetyl-1,4-dihydrolutidine (DDL) which forms a selective oxidation voltammetric peak at a potential of 0.4 V. Detection and quantification limits of 0.57 mg kg−1 and 1.89 mg kg−1 , respectively, were obtained, together with intra- and inter-day precision below 10% (as relative standard deviation, RSD). The developed methodology was applied to determine formaldehyde content in seven samples. Similar results were obtained from the European standard method EN 717-3 with a significant reduction of total analysis time. The developed method MLEM-SPCE, which combines the use of a new sample preparation procedure for volatile compounds with the firstly introduced determination of formaldehyde (as the derivative product DDL) on unmodified SPCEs, proves to be a promising alternative for the determination of formaldehyde in wood-based products and other samples.
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Determination of TauroLock stability
Matejčíková, Eva ; Mravcová, Ludmila (referee) ; Čáslavský, Josef (advisor)
This diploma thesis deals with the study of the anticoagulant and antimicrobial agent TauroLockTM, which is manufactured by the german company TauroPharm GmbH. Based on the background research and taking into account the equipment of analytical laboratories of the Institute of Chemistry and Technology of Environmental Protection, optimal methods of analysis were proposed and a series of experiments were carried out which focused on the determination of the active substance and degradation products of taurolidine in TauroLockTM which was stored for a certain period of time in real conditions. The main task of this work was to determine the presence of taurolidine as the active substance and formaldehyde as the undesirable product of its decomposition.
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The effect of fixatives on DNA quality and quantity in archival tissue specimen
Matura, Radan ; Vaněk, Daniel (advisor) ; Šolc, Roman (referee)
Formaldehyde is widely used fixative. Its advantages are low cost, simplicity of use and good fixation traits, which are fast tissue penetration, good preservation of morphological structures and compatibility with downstream histological applications. Formaldehyde disadvantages are negative effects on nucleic acids. Formaldehyde solutions modify primary structure of deoxyribonucleic acid (DNA), fragment DNA and create protein- DNA covalent bonds that hinder DNA isolation procedures. Level of negative effects of formaldehyde is dependent on many factors. Effect of formaldehyde chemical composition (formaldehyde dilution, presence of buffer or formic acid) and effect of fixation length were studied in this work. On DNA extracted from fixed tissues, DNA quantity and level of DNA fragmentation were studied by quantitative polymerase chain reaction, fluorescence assay for DNA quantification and by on-chip electrophoresis on bioanalyzer Agilent 2100. Quality and quantity of acquired DNA were tested by DNA profile determination for identification purposes using STR (short tandem repeats) analysis. Results show that of all tested fixatives, buffered 4% formaldehyde is the most suited solution in regards of sufficient amount of DNA and sufficient DNA quality. Other formaldehyde variants (non-buffered 4%...
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The effect of fixatives on DNA quality and quantity in archival tissue specimen
Matura, Radan ; Vaněk, Daniel (advisor) ; Šolc, Roman (referee)
Formaldehyde is widely used fixative. Its advantages are low cost, simplicity of use and good fixation traits, which are fast tissue penetration, good preservation of morphological structures and compatibility with downstream histological applications. Formaldehyde disadvantages are negative effects on nucleic acids. Formaldehyde solutions modify primary structure of deoxyribonucleic acid (DNA), fragment DNA and create protein-DNA covalent bonds that hinder DNA isolation procedures. Level of negative effects of formaldehyde is dependent on many factors. Effect of formaldehyde chemical composition (formaldehyde dilution, presence of buffer or formic acid) and effect of fixation length were studied in this work. On DNA extracted from fixed tissues, DNA quantity and level of DNA fragmentation were studied by quantitative polymerase chain reaction, fluorescence assay for DNA quantification and by on- chip electrophoresis on bioanalyzer Agilent 2100. Quality and quantity of acquired DNA were tested by DNA profile determination for identification purposes using STR (short tandem repeats) analysis. Results show that of all tested fixatives, buffered 4% formaldehyde is the most suited solution in regards of sufficient amount of DNA and sufficient DNA quality. Other formaldehyde variants (non-buffered 4%...
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Preventivní a léčebné koupele u plůdku candáta obecného (Sander lucioperca)
PITHARDT, Tomáš
The aim of this thesis was to test and compare the effect of four selected and in present commonly used medical treatments on other species of fish in recirculating aquaculture system (RAS). This presented work was divided into two separate experiments dealing with curative baths for fry of pikeperch.The fry was raised in ponds, feeded by natural food, with the intention of adaptating for RAS after a bath. Baths were targeted against parasitic and bacterial diseases by using Bellasav, SAVO Original, formaldehyde and Chloramine T. The curative baths took 30 minutes after which the fish were transferred to clean water where the mortality within 24 hours and the efficacy of the preparation within 24 hours was monitored. In the first experiment were used fish with an average weight of 0.23+-0.05 g and a total lenght of 31.7+-2.5 mm at the age of 35 days. The tested doses were 0.5; 1; 1.5; 2; 5; 10 and 50 ml.m-3 for Bellasav, 1; 5; 10; 20 and 50 ml.m-3 for SAVO Original, 0.5; 1; 1.5; 2.5; 5; 10; 15; 30; 45 and 50 ml.100 l-1 for formaldehyde and 10; 20; 30; 40; 50; 100; 150 and 200 mg.l-1 for Chloramine T. The best efficacy was achieved at the concentration dose 1.5 ml.m-3 for Bellasav. The concentration dose 10 ml.m-3 of SAVO Original only reduced parasitic infection by one degree. An effective dose of formaldehyde was 1 ml.100 l-1 which reduced the parasitic infection by two degrees. The most effective preparation was Chloramine T at the concentration dose of 30 mg.l-1. In the second experiment were used fish with an average weight of 1.6+-0.4 g and a total lenght of 60.4+-3.56 mm at the age of 70 days. The tested doses were 10; 20 and 50 ml.m-3 for Bellasav, 10; 20 and 50 ml.m-3 for SAVO Original, 2.5; 5 and 10 ml.100 l-1 for formaldehyde and 40; 150 and 200 mg.l-1 for Chloramine T. The best efficacy was achieved at the concentration dose 20 ml.m-3 for Bellasav and 10 ml.m-3 for SAVO Original. The most effective dose for formaldehyde was 2.5 ml.100 l-1 which reduced the bacterial infection in fish skin by two degrees. The most effective preparation was Chloramine T again at the concentration dose 40 mg.l-1.
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Fixation of the biological samples
Brachtl, Martin ; Jiřík, Radovan (referee) ; Krátká, Lucie (advisor)
The aim of thesis is acquaint with possibilities of fixation biological tissues. Choose a suitable tissue and fix it with formaldehyde and another fixator. Last but not least make measurements which determine effects of formaldehyde on the relaxation time of biological tissues. Mice brains were chosen as a suitable for tissues fixation. Measuring effects of formaldehyde and another fixator to relaxation time carried out at the Institute of Scientific Instuments Academy of Sciences of the Czech Republic.
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Premedical care for patients with methanol intoxication
KŘENEK, Jan
Intoxication through methanol is a relatively less frequent type of poisoning, which can lead even to a serious damage to the human body; can cause loss of vision as well as permanent infliction of the central nervous system. And also the death of the afflicted person is possible with late treated conditions. The methanol metabolites are quilted for the damage of the human body - mainly formic acid that is why the therapy of this health and life-threatening condition is mostly focused on the metabolism of methanol. The theoretical part deals with pre-medical that is pre-hospital emergency care of patients with a methanol intoxication. The first chapter deals with the pre-hospital emergency care, a medical rescuer and his competencies, and the ambulance crew is listed here as well. Furthermore, the theoretical part of the thesis deals with the acid-base balance, metabolic acidosis, but also involves the Toxicological Information Centre. The next chapter explore methanol and its characteristics and the history of methanol in the course of the human civilisation. It explains the metabolism of methanol and highlights the symptoms of acute and chronic methanol intoxications. The last chapter is directly focused on first aid in this state provided by a layman; and on the treat methanol intoxication in terms of pre-hospital emergency. Last but not least, it is also concerned with the effects of the methanol intoxication. The practical part of the thesis is focused on mapping the activities of a paramedic in a pre-medical care of a patient with a methanol intoxication and on determination the level of awareness of laity as well as the clients of the sobering-up station for this type of poisoning. As well as a laity with help of anonymous questionnaires. The research survey was conducted through the quantitative method among the paramedics. The amount of the data was collected during the March 2015. The research group were the randomly choired laymen and clients of the sobering-up station and the paramedics working in the Emergency Medical Services of South Bohemia Region at the regional centres of Strakonice, Písek, Prachatice, Český Krumlov and Jindřichův Hradec. The anonymous questionnaire was distributed to the above stated regional centres in the number of 100 pieces, for the laity in the same number and 10 pieces for the sobering-up station in České Budějovice. The questionnaire for the paramedics contained a total of 18 questions. The first four questions were the ones of stratification, the questions no 5 to 18 were closed and directly addressed the issues concerning the provision of pre-hospital emergency care in methanol intoxication, whereas the hypothesis no. 1 was confirmed. The questionnaire for the laity in itself contained a total of 14 questions. The first three questions were of socio demographic character. The fourth question was closed and crucial to the continued questionnaire. The question no. 5 was semi-open and remaining questions no. 6 to 14 related to determine the level of awareness among the laity methanol intoxication. The results are processed into clear graphs and the related hypothesis no. 2 was also confirmed. The first goal was to map the activity of a paramedic in a pre-medical care of a patient with a methanol intoxication. The second objective was to determine the level of awareness of the laity of this intoxication. Both the objectives have been met. The statistical method of processing a research survey showed that paramedics are knowledgeable in the procedures of pre-medical care for patients with a methanol intoxication and that lay people are informed of this intoxication, but would welcome more active involvement of experts in informing society about this issue. Based on the results of the work an information leaflet was produced for the lay and paramedics in the field of alcohol intoxication.
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Voda a životní prostředí
Inkoteka, Praha ; Nesvadba, Jindřich
Publikace se skládá ze dvou samostatných částí. První tvoří abecední slovník pojmů a jevů týkajících se vody ve vztahu k životnímu prostředí. Druhou tvoří studie o posuzování vlivu na životního prostředí v oblasti odpadového hospodářství. V této části jsou popsány typy posuzovaní vlivu na životní prostředí a ekobilancování jako diagnostická metoda. Jsou definovány indikátory pro sestavení programového EIA pro odpadové hospodářství. Studie rozebírá metody posuzování vlivu pro řízené skládky TKO, pro spalování odpadu a pro znovu. Závěrečná kapitola rozebírá posuzování různých systémových variant v oblasti odpadového hospodářství.
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